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Cellular Profiling Core Facility
Mass
Cytometry
Mass cytometry, or cytometry by time-of-flight, enables greater comprehension to your research by letting you simultaneously measure more than independent 40 parameters from millions of cells.
We house a federally-funded research facility committed to basic, applied and clinical research, discovery and collaboration to academic, biotech and pharmaceutical
industries.
A key component of the facility is a CyTOF mass cytometer.
Mass Cytometry
Mass cytometry uniquely combines time-of-flight mass spectrometry with Maxpar metal-labeling technology to enable breakthrough discovery and comprehensive functional profiling applications. Cellular targets are labeled with metal-tagged antibodies, detected and quantified by time-of-flight mass spectrometry. The high purity and choice of metal isotopes provide minimal background noise from signal overlap or endogenous cellular components. Up to 135 available detection channels ensure an ongoing ability to add more parameters, enabling you to fully study the functional complexity of biological systems at the single-cell level from populations of phenotyped cells.
Mass cytometry has catalyzed the revolution of single-cell proteomics, enabling the most comprehensive understanding of cell phenotypes, signaling pathways and function. We collaborate daily with top-level researchers to provide system-level biology at single-cell resolution, on an accessible expandable platform designed for breakthrough discovery.
Photos & Content: Fluidigm
How mass cytometry works
Discover your inner cell
DESIGN
Design high-dimensional proteomic panels with this interactive, web-based application that simplifies and optimizes panel design using metal-conjugated antibodies from the Fluidigm catalog and your custom conjugates.
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BUILD
The catalog contains over 400 metal-conjugated antibodies to human and mouse targets covering a wide range of applications including phenotyping, cytokine expression, signaling responses, apoptosis and cell cycle, plus a variety of pre-validated panel kits.
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STAIN
Maxpar™ Sample Prep Buffers and Fluidigm-validated protocols provide optimal mass cytometry staining performance for surface and intracellular targets. Use Cell-ID™ barcoding to increase throughput and decrease sample-to-sample staining variability.
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ACQUIRE & ANALYZE
Accelerate discovery through improved performance and a streamlined workflow, driven by a modern, intuitive user interface. Cytobank provides an array of analysis tools, including dot plots, clustering and dimensionality reduction algorithms
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THE ELEMENTS OF MASS CYTOMETRY
THROUGHPUT & RESOLUTION
Helios is barcoding enabled. This increases throughput while improving data quality, allowing you to analyze more experimental variables simultaneously.
METAL ISOTOPES
Mass cytometry employs heavy metal isotope tags to simultaneously measure multiple cellular targets. High-purity metallic isotopes ensure minimal background from signal overlap or endogenous cellular components.
CHANNELS
Helios expands the mass cytometry range to 135 channels ensuring comprehensive studies today, and flexibility to scale in the future as new metal tags are developed.
PANELS
Fluidigm offers metal-tagged antibodies as individual reagents and in preassembled kits targeting specific applications, which can be combined to build high-dimensional proteomic panels for profiling cellular systems.
DISCOVERY WORKFLOW WITH MASS CYTOMETRY
MASS CYTOMETRY ELEMENTS
The stable isotopes of these 24 elements provide over 50 unique tags for use in mass cytometry experiments.
DESIGN
BUILD
STAIN
ACQUIRE
ANALYZE
