External links to other sites outside of the our domain are being provided as a convenience and for informational purposes only; they do not constitute an endorsement or an approval by Rogers Laboratory Cellular Profiling Core Facility of any of the products, services or opinions of the corporation or organization or individual. Rogers Laboratory Cellular Profiling Core Facility bears no responsibility for the accuracy, legality or content of the external site or for that of subsequent links. Contact the external site for answers to questions regarding its content.
Our facility does not have any financial arrangement with the resources listed below. We have gathered these links and present them here because we have found information within those pages useful and beneficial to our work and research success.
Cytoforum
The Cytoforum is the best place to connect with the greater CyTOF community on a variety of topics ranging from experimental design, instrument operation, data analysis and more. Search older posts, ask your questions, get information on publications, and answer questions in your area of expertise.
An antibody panel design tool and supporting documents are available by creating an account with Fluidigm. Please be sure to read the Panel Design User Guide before utilizing the design tool.
Pre-conjugated antibodies are available from Fluidigm and from the BWH Antibody Core. Fluidigm also providespre-designed panel kits, sample prep reagents, barcoding kits. and Max-Par antibody conjugation kits to conjugate your own antibodies.
Protocols for antibody conjugation, cell surface, viability, cytoplasmic targets, nuclear targets, signaling proteins and phosphoproteins can be found on Fluidigm’s website by scrolling down to the CyTOF section. They can also be accessed when logged into the panel design tool website (see the panel design button above).
Like Fluidigm, the Lederer Lab at Brigham and Women’s Hospital offers an expansive catalog of metal tagged antibodies. We can offer guidance in constructing a panel that will optimize coverage and resolution.